Biocytin-filled
WebMar 1, 2024 · For imaging biocytin-filled GCs and the neurochemical phenotypes of transplanted cells, we collected optical slices through the entire thickness of 350-μm-thick fixed and stained hippocampal slices that had been used for electrophysiological studies. Confocal microscopy (Zeiss LSM 510 or a Leica SP8) was performed at 0.5 μm steps … WebSep 29, 2014 · Importantly, however, reconstruction of biocytin-filled PTEN KO cells occasionally revealed multiple axon collaterals projecting into CA3 (2 of 6 neurons), while all control cells examined had only a single collateral in this region (n=5). Mossy fiber sprouting, in which granule cell axons project back into the molecular layer, was also observed.
Biocytin-filled
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WebOct 12, 2024 · b Representative images of neurons for the recordings in the VTA and the neuron filled with dye. Scale bar, 20 μm. ... o Responsive Mor-Ens from slice of mice treated with saline or escalating dose of morphine was filled with biocytin and stained with anti-TH antibody after recording to identify the TH + subtypes (Saline: n = 24 neurons … WebJul 15, 2015 · Biocytin filled relay cells in dLGN of WT and math5 −/−. Examples of relay cells arranged by postnatal week (1–5) for WT (left panel) and math5 −/− (right). Three-dimensional reconstructions are based on Z-stack images of representative relay neurons filled with biocytin. Arrowhead points to the axon. Scale bar = 58 μm
WebNov 16, 2011 · Biocytin-filled cells were incubated with peroxidase-avidin-biotin complex (ABC, Vector Laboratories) and visualized with DAB-nickel sulfate. Subsequently, slices were mounted onto microscope slides and coverslipped with Mowiol. The biocytin-filled neuron reconstruction was performed with the aid of a Neurolucida 3D reconstruction … WebDec 31, 2016 · We show that thick biocytin-filled sections that were stained and coverslipped can be restained with a second primary antibody days or months later. …
WebImmunostaining Of Biocytin-filled And Processed Sections For Neurochemical Markers. JoVE publishes peer-reviewed scientific video protocols to accelerate biological, medical, chemical and physical research. Watch our scientific video articles. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific … WebAfter localization of the biocytin-filled neurons, the slides containing the appropriate sections are processed for the presence of TH using fluorescence immunohistochemistry.We utilize several electrophysiological criteria together with the anatomical location (slices 7, 8, 9 and rostral part of slice 10; Fig. 1) for assigning a cell …
WebSep 12, 2007 · Biocytin-filled, KCC2-positive cells are yellow in the merge. Red arrowheads indicate the soma, and open arrowheads indicate the apical dendrite. Confocal fluorescent images of 5–10 μm stack thickness are shown. A, Six of six hyperpolarized cells were immmunopositive for KCC2.
WebNov 20, 2024 · The protocol outlined here describes how biocytin-filled cells recorded in the CA2 region of the hippocampus are recovered and then reconstructed in 3D. … gr 11 term 4 2019 efal literature summary.pdfWebIn more details, a biocytin (C 16 H 28 N 4 O 4 S) self-assembled monolayer is adsorbed onto the base of a CaF 2 prism closing a Teflon cell. The latter is then filled with a HEPES buffer solution containing test proteins: avidin, avidin presaturated with biotin (PSA), or bovine serum albumin (BSA). After 16 h of contact with the biocytin film ... gr 11 physical science term 2 exam juneWebThis biocytin filled neuron is a fast-spiking, parvalbumin-expressing GABAergic basket cell in the CA1 region of the mouse hippocampus. Dendrites (blue) of this cell type … gr 11 trigonometry notesWebMay 30, 2002 · We report, that this method facilitates the identification of intracellular organelles of the biocytin-filled neuron and of postsynaptic densities. This method is valid for the study of early postnatal neurons that are particularly refractory to this type of analysis. The procedure introduced here consists of the following steps: (1) injection ... gr 11 physics past paperWebAug 7, 2009 · Simultaneous responses are shown from both locations, with increasing stimulation intensity near the blue electrode (B) and the red electrode (C). (D) Reconstruction of a biocytin-filled L5 pyramidal neuron showing the experimental setup. Dendritic recording pipettes are shown near the third bifurcation (875 μm from the soma, … gr 1275 core downloadWebJul 28, 2024 · (H) Closest apposition of the biocytin filled bouton and IN dendrite is shown in a single 200-nm-thin section. White outlines help distinguishing the two profiles throughout the immunolabeled images. (I) The same section was immunolabeled for vGluT1, PSD95, Munc13-1, Bassoon, and Piccolo and for AMPA receptors (panAMPAR Ab) in 3 labeling … gr11 physics past papersWebMay 9, 2016 · Biocytin-filled neurons were morphologically identified as SPNs post-hoc by the presence of dendritic spines (Figure 2e, right, white arrows). Eight out of 16 neurons were recovered after patching and were processed for imaging; all eight of these neurons showed a high density of dendritic spines at 40-63X magnification. gr1 26l backpack