WebWhat is claimed is: 1. A set of primers for performing multiplex RNA sequencing, comprising 4 sets of oligonucleotides, including: (1) An RT primer for capturing an mRNA and synthesizing a 1 st strand of cDNA; (2) A 2 nd strand cDNA synthesis primer for 2 nd strand cDNA synthesis starting from multiple sites along the 1 st strand of cDNA; (3) A PCR … WebJan 22, 2024 · Library preparation and sequencing. Single-cells were sorted in 2uL of Lysis Buffer (0.2% v/v Triton X-100 (Sigma-Aldrich, cat. no. T9284) with 2U/ul RNase Inhibitor …
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WebJan 2, 2014 · 5. Place each single cell into a 0.2-ml thin-walled PCR tube containing 2 μl of cell lysis buffer, 1 μl of oligo-dT primer and 1 μl of dNTP mix. 6. Quickly vortex the tube … Web1μg of Lambda DNA/HindIII Markers is incubated with 200 units of Ribonuclease Inhibitor for 16 hours at 37°C. Following incubation, Lambda DNA/HindIII Markers is separated by 1% agarose gel and stained with ethidium bromide. Markers remain as intact bands without smearing. To test for the presence of RNase activity, 1ug of RNA is incubated ... rooting compound for grapes
MustSeq, an alternative approach for multiplexible strand-specific …
WebProtocol No. PT4052-5 www.clontech.com Clontech Laboratories, Inc. Version No. PR073572 3. pLVX-shRNA2 Vector Information. Notice to Purchaser. Clontech products are to be used for research purposes only. They may not be used for any other purpose, including, but not limited . to, use in drugs, in vitro. diagnostic purposes, therapeutics, or … WebClontech products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of Clontech Laboratories, Inc. cPPT Element: This product and its use are the subject of U.S. Pat. No. 6,682,907 and pending foreign patent ... WebIn addition, because it is a protein, it differs from other competitive inhibitors (nucleotides and inorganic phosphates) by being able to be easily removed from the reaction system … rooting city