Web1 µL of FastDigest BamHI is formulated to digest up to: – 1 µg of lambda DNA in 5 min. – 1 µg of plasmid DNA in 5 min. – 0.2 µg of PCR product in 5 min. – 1 µg of genomic DNA in … WebJan 18, 2010 · Now, I have to digest them with Fast digest ® BamHI and XhoI enzymes (Fermentas). In Fermentas manual, it was mentioned that these Fast digest enzymes will cut the DNA in 15 min. Once, I subjected digestion for 4 hours at 37⁰C. For, pET 15b, I observed several bands.

High-Fidelity (HF®) Restriction Endonucleases NEB

WebCut at G^GATCC sites with Thermo Scientific™ FastDigest™ BamHI restriction enzyme, which performs best at 37°C in 5-15 minutes using universal FastDigest Buffer. ... FastDigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest and FastDigest Green reaction buffers. WebExperts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!* See Answer *Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers. ... Given: BamHI, cleaves after the first G: 5’ G GATCC 3’ 3’ CCTAG G 5’ AND BclI ... dr brent decker psychologist panama city

FastDigest BamHI

Web2 days ago · The PCR products were digested by BamHI and PstI (New England Biolabs, MA, USA), and ligated to predigested pMo130TFR. ... USA) and Random Hexamer Primer (Thermo Fisher Scientific, MA, USA). qPCR ... WebBSA is supplied at 10X concentration; for use, dilute 10-fold to obtain a final concentration of 0.01%. Notes: Ten units of each enzyme completely digests 1 µg of DNA at 37°C in one hour in 50 µl reaction mixture. The concentration of glycerol should be less than 10% to minimize star activity. WebBamHI G↓GATCC Unique 20-50* 100 20-50 50-100* 100* 50-100 1X* or 2X BauI (BssSI) CACGAG(-5/-1) ... dr. brent cole bethesda md