Webb11 jan. 2016 · SAMtools不仅仅用来call snp。从samtools的软件名就能看出,是对SAM格式文件进行操作的工作,比如讲sam转成bam格式,index,rmdup等等。samtools结合linux命令比如grep,awk和SAM格式描述的flag,tag,亦是非常非常非常强大,比如根据flag过滤duplicate的reads,根据XA tag过滤multiple hit的reads。 Webb30 juli 2024 · 1 HISAT2官网下载 人类和小鼠的索引有现成的, HISAT2官网 可以直接下载进行序列比对。 如下图所示:选择hg19和mm10的index,文章中RNA-Seq测序数据,可以包括人类和小鼠的数据,因此需要小鼠和人类的索引。
Main HISAT2
Webb15 juni 2024 · Introduction. HISAT2 is the fastest spliced mapper currently available. It is part of the new tuxedo suite of tools and it will map RNA-Seq data to the genome as well as identify splice junctions. HISAT2, like BWA and bowtie, uses burrows-wheeler transform (BWT) to compress genomes such that they require very little memory to store. Webb2 aug. 2024 · Bowtie2 required the smallest amount of memory (3.4 GB), followed by HISAT2.Linear (4.5 GB) and BWA-mem (5.7–6.2 GB). Graph-based aligners (HISAT2.Graph and vg) required more RAM, with HISAT2 ... book shopping clipart
RNA-seq分析之hisat2--建立index - 知乎
http://findelephant.com/sheng-wu-xin-xi-xue-bi-ji-4-hisat2-de-xia-zai-an-zhuang-ji-huan-jing-pei-zhi.html Webb16 mars 2024 · Overview. HISAT2 is a fast and sensitive alignment program for mapping next-generation sequencing reads (whole-genome, transcriptome, and exome … Webb17 mars 2024 · conda第一步,你确定安装成功了吗? 2 安装过一款软件,我特别想知道他都载入了环境什么命令,能不能从服务器上查询到,这样的话我就不用记住安装过哪个软件,需要调取哪个命令的帮助文档啦? book shopping cart