WebThe copA antisense RNA determines the incompatibility group to which the plasmid belongs; thus, copA is also referred to as incRNA 18. The second element that controls plasmid copy number of... WebDec 31, 2011 · The presence of plasmid-mediated quinolone resistance (PMQR) genes and β-lactamase genes were established by PCR amplification. PCR-based replicon typing (PBRT) revealed that these 32 isolates represented seven plasmid incompatibility groups (IncP, HI2, A/C, FIIs, FIA, FIB, and I1), and the IncHI2 (59.4%) was predominant.
Incompatibility of plasmids containing the replication origin of the ...
WebMar 15, 2016 · Plasmid analysis Plasmids were classified according incompatibilitygroups using PBRTscheme described Caratolliet al. Primerswere obtained from Thermo Fisher Scientific (Ulm, Germany). ... PBRTidentified six different incompatibility groups: IncFII (n31); IncFIB (n26); IncI1 Portugalhad highestnumber leastone replicon type (n13), … WebPlasmids of the same incompatibility group have very similar DNA sequences in their replication and partition genes, although the other genes they carry may be very different. … citibank cosigner credit card
Plasmid typing and genetic context of AmpC β-lactamases in ...
WebApr 13, 2024 · The emergence of carbapenem-resistant Klebsiella pneumoniae poses a significant threat to public health. In this study, we aimed to investigate the distribution and genetic diversity of plasmids carrying beta-lactamase resistance determinants in a collection of carbapenem-resistant K. pneumoniae blood isolates. Blood isolates of … WebJan 1, 2024 · Here we analyse the mechanism of partition-associated incompatibility for plasmid pB171, a phenotype associated with all known plasmid-encoded centromere loci. An R1 plasmid carrying par2 from ... WebMay 23, 2012 · It turns out that there doesn't seem to be a specific mechanism to prevent multiple incompatible plasmids from coexisting.Velappan et al put in more than one incompatible plasmid with different selection genes in them and put the bacteria on a single antibiotic and periodically checked for the second vector (by testing for second antibiotic … citibank corporate telephone number