WebFeb 23, 2011 · Modifications from the reported procedure include: a decrease in the detritylation time to 1 min, as this step was closely monitored by UV analysis for complete release of the trityl group; phosphoramidite concentration was 0.1 M; 4,5-dicyanoimidazole catalyst was used at 0.7 M in the coupling step; 3-picoline was used instead of pyridine … WebMar 29, 2024 · The phosphorothioate bond oligo modification alters the phosphate linkage by replacing one of the non-bridging oxygens with a sulfur atom. This alteration of the …
Mechanisms of single-stranded phosphorothioate modified …
WebAug 31, 2016 · Indeed, a decrease in transformation rate was observed, comparing between a phosphorothioate-modified plasmid and its non-modified counterpart in Salmonella enterica (Xu et al., 2010). A DNA damaging phenotype was also seen when the modification gene cluster was knocked out, and several genes downstream of the dnd genes were … WebSpecificity Enhancement of PCR and Other DNA Polymerization Using Phosphorothioate-Modified dNTPs PCR nucleic acids Posted in: Nucleic Acids, Diagnostics Up to 100-fold … imdb the lives of others
Mechanisms of single-stranded phosphorothioate modified …
Webnoun. phos· pho· ro· thio· ate ˌfäs-fə-rō-ˈthī-ō-ˌāt. : an oligonucleotide in which the oxygen atom normally linking two consecutive nucleotides has been replaced with sulfur and which resists degradation by cellular enzymes compare phosphodiester. WebFeb 23, 2011 · An abasic phosphorothioate deoxyribose oligomer (N = 20) failed to compete for antisense activity suggesting that the hydrophobic bases may contribute to cell uptake … WebPhosphorothioate modification is frequently used to enhance the nuclease resistance of oligonucleotide. 23 Therefore in this study, to further improve the nuclease-resistance of Apt-HJ, the first nucleotide at 5ʹ-end of each DNA strand was modified with phosphorothioate. To assess the serum stabilities of phosphorothioate-modified and non … imdb the long night